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Basic base in organic chemistry
Jan 10, 2018

Amino (Amino) is the basic base in organic chemistry, all amino-containing organic substances have a certain base characteristics, by a nitrogen atom and two hydrogen atoms, the formula -NH2. Such as amino acids on the amino group, there is a certain alkaline characteristics. Amino groups are large, easily oxidized groups. In organic synthesis, it is necessary to protect with easily removable groups. Simply put several: 1, acylation protection, that is, with anhydride protection 2, with benzyl protection 3, chiral compounds commonly used CBZ, BOC, FMOC protection of amino acids

Aminotransferases, also known as transaminases, catalyze the a-keto exchange of the amino group of an a-amino acid with an a-keto acid, a function known as transamination. It plays an important role in the metabolism and metabolism of sugar, fat and protein in the intermediate metabolism of protein synthesis and decomposition in vivo. When any amino acid is transaminated, it is catalyzed by its specific aminotransferase. Their optimum pH is close to 7.4. Of the various aminotransferases, glutamic oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) have the strongest activity.

1/15 mol / L phosphate buffer (pH = 7.4), 1% glutamic acid solution (neutralized to neutral with KOH), 1% pyruvic acid solution (neutralized to neutral with KOH), 0.1% Solution, 0.05% iodoacetic acid solution, 15% trichloroacetic acid solution, standard alanine solution (0.1%), standard glutamic acid solution (0.1%), 0.1% ninhydrin ethanol solution,

The rabbit stunned, quickly dissected, remove the liver, cut at low temperatures. Weigh homogenate, the supernatant was centrifuged, that is, the preparation of the enzyme solution. In vitro amino exchange reaction. Four spots per person were examined by paper chromatography (standard alanine solution, standard glutamic acid solution, control supernatant, sample supernatant). Point into the chromatography cover. With phenol saturated aqueous solution, after the balance, with a small funnel glass tube from the chromatographic cap saturate phenol reagent 25ml in the chromatographic cylinder. Immediately thereafter, close the plug. When the solvent front reaches about 2.5 cm from the upper end of the filter paper, remove the filter paper and allow to dry or blow dry to remove the phenol solvent. Spray 0.1% ninhydrin in ethanol, Ninhydrin reaction, showing purple spots on the filter paper.

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